Endoproteinase Asp-N; Excision Grade; Pseudomonas fragi

Code: 324708-2UG D2-231

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural doma...


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€329.89 inc. VAT

General description

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Packaging

2 µg in Plastic ampoule

Physical form

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Unit Definition

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL™ Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Warning

Toxicity: Harmful (C)

assay≥90% (SDS-PAGE)
colorwhite
formlyophilized solid
manufacturer/tradenameCalbiochem®
Quality Level100
shipped inambient
solubilitywater: soluble
specific activity≥20,000 units/mg protein
storage conditionOK to freeze
storage temp.2-8°C
Cas Number9001-92-7
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